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2.8 Lipid peroxidation assessment Malondialdehyde (MDA), a stable end product of lipid peroxidation during oxidative stress and cellular damage was measured using the Thiobarbituric Acid Reactive Substances (TBARS) assay ( 2.9 Measurement of nitric oxide production Griess reagent (Sigma Aldrich, USA) was utilized to determine the concentration of NO ( 2.10 Measurement of hydrogen sulphide H 2 S released from the hepatic and pancreatic tissues of mice were evaluated by methylene-blue assay (Smith and Pluth, 2023)